Method for expansion of human corneal endothelial cells

Details Method for expansion of human corneal endothelial cells Method for expansion of human corneal endothelial cells

A - Human Necessities

61

F

A61F 9/007 (2006.01) A61K 35/54 (2006.01) A61L 27/38 (2006.01) C12N 11/00 (2006.01) C12N 5/08 (2006.01)

Patent

CA 2650579

A method for expanding human corneal endothelial cells includes: (a) providing an amniotic membrane with or without amniotic cells, wherein the amniotic membrane has an extracellular matrix; (b) placing onto the amniotic membrane, a sheet of endothelial layer, or a cell suspension including human corneal endothelial stem cells; and (c) culturing the corneal endothelial cells on the amniotic membrane for a duration sufficient for the corneal endothelial stem cells to expand to an appropriate area. The invention also relates to a method for creating a surgical graft for a recipient site of a patient using the method for expanding human corneal endothelial cells, and the surgical graft prepared therefrom.

L'invention concerne un procédé permettant l'expansion de cellules endothéliales cornéennes humaines, qui comprend les étapes consistant à (a) proposer une membrane amniotique avec ou sans cellules amniotiques, dans lequel la membrane amniotique comporte une matrice extracellulaire ; (b) placer sur la membrane amniotique une feuille de couche endothéliale ou une suspension de cellules comprenant des cellules souches endothéliales cornéennes humaines ; (c) cultiver les cellules endothéliales cornéennes sur la membrane amniotique pendant une durée suffisante pour que les cellules souches endothéliales cornéennes se dilatent jusqu'à une zone appropriée. L'invention concerne également un procédé de création d'une greffe chirurgicale pour un site récepteur d'un patient utilisant le procédé d'expansion de cellules endothéliales cornéennes humaines, et la greffe chirurgicale préparée à partir de celles-ci.

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