Method for exponential amplification of nucleic acid by a...

Details Method for exponential amplification of nucleic acid by a... Method for exponential amplification of nucleic acid by a...

C - Chemistry, Metallurgy

12

N

C12N 15/10 (2006.01) C12Q 1/68 (2006.01) C12Q 1/70 (2006.01)

Patent

CA 2123686

A process for exponentially amplifying a selected nucleic acid sequence present in a sample, comprising the steps of forming a mixture of the sample and a single primer designed to hybridize with the selected nucleic acid sequence; causing the single primer to hybridize to a single strand of the nucleic acid sequence of interest; forming a duplex product of the nucleic acid by a polymerase reaction; separating the duplex product into single strands; and repeating the preceding steps until the rate of production of the amplification product is exponential and the nucleic acid sequence of interest has been amplified.

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