Method for exposing group a streptococcal antigens and an...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/24 (2006.01) C12Q 1/00 (2006.01) C12Q 1/14 (2006.01) G01N 33/536 (2006.01) G01N 33/543 (2006.01) G01N 33/569 (2006.01)

Patent

CA 1301645

ABSTRACT OF THE DISCLOSURE The present invention relates to the rapid detection of Group A streptococci in clinical specimens, through the enzymatic digestion by a semi-purified Group C streptococcal phage associated lysin enzyme and the identification of the released antigens, through the reaction of a labelled ligand and its respective antigen or receptor. The labelled ligand can be included during the digestion of the bacteria, enabling the total assay time to be less than five minutes. The lysin enzyme is stabilized and can be lyophilized for in situ reconstitution.

548939

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