Method for filtering nucleic acids, in particular from fixed...

Details Method for filtering nucleic acids, in particular from fixed... Method for filtering nucleic acids, in particular from fixed...

C - Chemistry, Metallurgy

12

N

C12N 15/10 (2006.01)

Patent

CA 2728484

The invention relates to a method for filtering nucleic acids, to a kit for carrying out the method according to the invention and to a novel use of magnetic particles for filtering a biological sample. The method according to the invention comprises the following steps: a) the sample is held in an aqueous solution; b) lysing of the sample; c) separation of cellular debris; and d) the nucleic acids are isolated from the solution, steps (a) to (c) taking place under non-chaotropic conditions.

La présente invention concerne un procédé de purification d'acides nucléiques, un kit pour mettre en oeuvre ledit procédé, et une nouvelle utilisation de particules magnétiques pour purifier un échantillon biologique. Ledit procédé consiste (a) à introduire l'échantillon dans une solution aqueuse, (b) à lyser l'échantillon, (c) à éliminer les débris cellulaires, et (d) à isoler les acides nucléiques de la solution, les étapes (a) à (c) ayant lieu dans des conditions non chaotropiques.

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