Method for freezing engrafting cells

A - Human Necessities – 01 – N

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A01N 1/02 (2006.01) A61K 35/14 (2006.01) A61K 35/28 (2006.01)

Patent

CA 2122140

2122140 9307745 PCTABS00021 The present invention provides a method for preparing engrafting cells for future use, comprising the steps of (a) purifying engrafting cells; (b) concentrating the purified engrafting cells; (c) resuspending the concentrated cells to a concentration of about 10 x 106 to 40 x 106 cells/ml in a solution that substantially maintains cell viability during freezing; and (d) freezing the resuspended cells under a first set of conditions which substantially maintains cell viability.

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