Method for gene identification signature (gis) analysis

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) C12N 15/00 (2006.01)

Patent

CA 2538137

An isolated oligonucleotide comprising at least one ditag, wherein the ditag comprises two joined first and second sequence tags, wherein the first tag comprises the 5'-terminus sequence and the second tag comprises the 3'- terminus sequence of a nucleic acid molecule or a fragment thereof. The ditag analysis is useful for gene discovery and genome mapping.

L'invention concerne un oligonucléotide isolé comprenant au moins une double étiquette composée deux étiquettes jointes d'une première séquence et d'une deuxième séquence, la première étiquette contenant la séquence de terminaison 5' et la deuxième étiquette la séquence de terminaison 3' d'une molécule d'acide nucléique ou d'un fragment de celle-ci. L'analyse de la double étiquette sert à la découverte de gènes et à la cartographie génomique.

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