Method for gene transfer to the central nervous system

A - Human Necessities – 61 – K

Patent

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Details

A61K 35/12 (2006.01) A61K 31/7088 (2006.01) A61K 48/00 (2006.01) A61P 35/00 (2006.01) C12N 15/867 (2006.01)

Patent

CA 2251232

The present invention relates to a method for implanting producer cells into the mammalian brain. The producer cells are engineered with a retroviral based recombinant vector encoding a tumorcidal factor or susceptibility factor that confers on tumor cells sensitivity to chemotherapeutic or radiotherapeutic agents. Prior to transplantation into the mammalian brain, the producer cells are first cultured in vitro on a support matrix to increase the long-term viability of the transplanted cells and to provide long-term functional benefit.

Procédé d'implantation de cellules productrices dans le cerveau d'un mammifère. Les cellules productrices ont subi une transformation par un vecteur de recombinaison basé sur un rétrovirus codant un facteur de nécrose tumorale ou un facteur de susceptibilité conférant aux cellules tumorales la sensibilité voulue aux agents chimiothérapeutiques ou radiothérapeutiques. Avant leur transplantation dans le cerveau du mammifère, les cellules productrices sont tout d'abord cultivées in vitro sur une matrice-support, pour accroître la viabilité à long terme des cellules transplantées et obtenir des avantages fonctionnels à long terme.

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