Method for generating active antibodies against a resistance...

A - Human Necessities – 61 – K

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A61K 39/00 (2006.01) C07K 16/00 (2006.01) C07K 16/30 (2006.01)

Patent

CA 2663561

The present invention relates to the use of a milled homogenate and/or a suspension and/or a cell lysate, stemming from a tumor resistant to at least one anti- tumoral compound in order to immunize and generate in vitro an antibody, or one of its functional fragments, directed against a tumoral antigen specifically expressed at the surface of said resistant tumor and being possibly involved in the resistance of said resistant tumor. More particularly, the present invention is directed to such antibodies obtained by applying the method, such as the antibodies 1A6, 1A9, 2E11, 3C11 and 3G7, as well as to their use for treating cancer.

L'invention concerne l'utilisation d'un produit de broyage et/ou d'une suspension et/ou d'un lysat cellulaire provenant d'une tumeur résistante à au moins un composé anti-tumoral à des fins d'immunisation et de génération in vitro d'un anticorps, ou d'un de ses fragments fonctionnels, pour lutter contre un antigène tumoral spécifiquement exprimé à la surface de la tumeur résistante et pouvant éventuellement intervenir dans la résistance de la tumeur résistante. L'invention porte en particulier sur des anticorps obtenus par ce procédé tels que les anticorps 1A6, 1A9, 2E11, 3C11 et 3G7, ainsi que sur leur utilisation pour traiter le cancer.

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