Method for generating amplified rna

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) C07H 21/04 (2006.01) C12P 19/34 (2006.01)

Patent

CA 2476661

The present invention provides a new method for producing amplified RNA from a selected set of cells. This method combines and utilizes known methods in a novel way to produce the gene expression profiles. The methods of the present invention utilize thermostable DNA polymerases and RNase H to produce high fidelity second strand synthesis of nucleic acids from selected cells in a highly efficient manner and in substantially reduced time.

La présente invention concerne un nouveau procédé pour produire l'ARN amplifié à partir d'un ensemble sélectionné de cellules. Ce procédé combine et utilise des procédés connus d'une façon nouvelle pour produire des profils d'expression génique. Les procédés de la présente invention utilisent des RNase H et polymérases d'ADN thermostables pour permettre la synthèse du deuxième brin haute fidélité d'acides nucléiques à partir des cellules sélectionnées d'une manière hautement efficace et dans un délai sensiblement réduit.

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