Method for generating aptamers with improved off-rates

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/115 (2010.01) A61K 31/7088 (2006.01) C07H 21/00 (2006.01) C12N 15/10 (2006.01) C12Q 1/68 (2006.01) C40B 30/04 (2006.01) C40B 40/06 (2006.01)

Patent

CA 2696431

The present disclosure describes improved SELEX methods for producing aptamers that are capable of binding to target molecules and improved photoSELEX methods for producing photoreactive aptamers that are capable of both binding and covalently cross linking to target molecules. Specifically, the present disclosure describes methods for producing aptamers and photoaptamers having slower dissociation rate constants than are obtained using prior SELEX and photoSELEX methods. The disclosure further describes aptamers and photoaptamers having slower dissociation rate constants than those obtained using prior methods. In addition, the disclosure describes aptamer constructs that include a variety of functionalities, including a cleavable element, a detection element, and a capture or immobilization element.

La présente invention concerne des procédés SELEX améliorés pour produire des aptamères qui sont capables de se lier à des molécules cibles et des procédés photoSELEX améliorés pour produire des aptamères photoréactifs qui sont capables à la fois de se lier et de se réticuler de manière covalente à des molécules cibles. Précisément, la présente invention concerne des procédés pour produire des aptamères et des photoaptamères ayant des constantes de vitesse de dissociation plus lentes que celles obtenues en utilisant les procédés SELEX et photoSELEX antérieurs. L'invention concerne en outre des aptamères et des photoaptamères ayant des constantes de vitesse de dissociation plus lentes que celles obtenues en utilisant des procédés antérieurs. En plus, l'invention concerne des constructions d'aptamères qui comprennent une variété de fonctionnalités, y compris un élément clivable, un élément de détection et un élément de capture ou d'immobilisation.

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