Method for generating cloned animals using chromosome shuffling

A - Human Necessities – 01 – K

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A01K 67/027 (2006.01) C12N 5/06 (2006.01) C12N 15/00 (2006.01) C12N 15/85 (2006.01) C12N 15/87 (2006.01) G01N 33/00 (2006.01)

Patent

CA 2425100

The present invention concerns the use of chromosomal replacement techniques in the context of producing cloned and transgenic animals, in order to correct chromosome abnormalities or alter autosomal genotypes, and provide for novel breeding pairs by replacing the sex chromosome in animals to be cloned. Replacement of a sex chromosome, or an X or Y chromosome, will result in animals that are autosomally isogenic and sexually non-isogenic (AISN), with "autosomally isogenic" meaning that the paired sets of autosomes (non-sex chromosomes) in each animal are isogenic or identical. Also included in the invention are animals that are both "autosomally" and "allelically" isogenic whereby each particular pair of chromosomes is internally isogenic or identical within a single animal as well as between animals. Such animals are particularly useful in generating a line of cloned mammals using sexual reproduction, without having the undergo nuclear transfer in order to propagate cloned animals.

La présente invention concerne l'utilisation de techniques chromosomiques de remplacement dans le contexte de production d'animaux clonés et transgéniques. Ces techniques visent à corriger des anomalies chromosomiques ou à modifier des génotypes autosomiques, et à fournir de nouveaux couples reproducteurs par remplacement du chromosome sexuel chez des animaux à cloner. Le remplacement d'un chromosome sexuel, ou d'un chromosome X ou Y, a pour résultat des animaux qui sont autosomiquement isogéniques et sexuellement non isogéniques (AISN), l'expression <= autosomiquement isogéniques >= signifiant que les ensembles appariés d'autosomes (chromosomes non sexuels) dans chaque animal sont isogéniques ou identiques. L'invention concerne également des animaux qui sont à la fois isogéniques <= autosomiquement >= et <= alléliquement >=, chaque paireparticulière de chromosomes étant intérieurement isogénique ou identique aussi bien chez un même animal que d'un animal à l'autre. De tels animaux sont particulièrement utiles pour créer une lignée de mammifères clonés par reproduction sexuelle, sans avoir à effectuer de transfert nucléaire pour augmenter le nombre d'animaux clonés.

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