Method for generating diversity

Details Method for generating diversity Method for generating diversity

C - Chemistry, Metallurgy

12

N

C12N 5/07 (2010.01) C07K 16/00 (2006.01) C12N 15/01 (2006.01) C12N 15/09 (2006.01) C12N 15/13 (2006.01) C12P 21/00 (2006.01) C12P 21/08 (2006.01) C12Q 1/02 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2449264

The invention relates to a method for preparing an antibody-producing cell line capable of directed constitutive hypermutation of a specific nucleic acid region, comprising the steps of: a) screening a clonal cell population for V gene diversity; b)isolating one or more cells which display V gene diversity and comparing the rate of accumulation of mutations in the V genes and other genes of the selected cells; and c) selecting a cell in which the rate of V gene mutation exceeds that of other gene mutation.

L'invention concerne un procédé permettant de préparer une lignée cellulaire productrice d'anticorps, capable d'une hypermutation constitutive dirigée d'une région spécifique d'acide nucléique, qui consiste a) à explorer une population cellulaire clonale pour une diversité des gènes V; b) à isoler au moins une cellule affichant une diversité des gènes V et comparer le taux d'accumulation des mutations dans les gènes V et dans d'autres gènes des cellules choisies, et c) à choisir une cellule dans laquelle le taux de mutation des gènes V dépasse celui de la mutation d'autres gènes.

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