C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01) C12N 9/38 (2006.01) C12N 15/00 (2006.01) C12N 15/10 (2006.01) C12P 19/34 (2006.01)
Patent
CA 2422749
A novel method for producing recombinant polynucleotides in vitro is provided. This method entails the treatment of heteroduplex DNA sequences with a nuclease (preferably, DNase I) and a polymerase (preferably, DNA polymerase I), the enzymes primarily involved in nick translation. The results achieved using this process are superior to that achieved by previous in vivo recombination efforts utilizing specific DNA repair systems.
L'invention porte sur un nouveau in vitro consistant à traiter des séquences d'ADN hétéroduplex par une nucléase (de préférence une polymérase I d'ADN), les enzymes étant d'abord impliquées dans une translation de coupure. Les résultats obtenus avec cette procédure sont supérieurs à ceux des recombinaisons in vivo antérieures utilisant des systèmes spécifiques de réparation d'ADN.
Bernstein Jeffrey
Mccarthy James K.
Moore Jeffrey C.
Merck & Co. Inc.
Ogilvy Renault Llp/s.e.n.c.r.l.,s.r.l.
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