C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/10 (2006.01) C07K 14/435 (2006.01) C12N 9/10 (2006.01) C12N 9/12 (2006.01) C12N 9/88 (2006.01) C12N 15/62 (2006.01) C12N 15/64 (2006.01) C12N 15/82 (2006.01) C12P 21/02 (2006.01)
Patent
CA 2374497
A new type of transgene system is disclosed which allows efficient protein expression in a target host such as a plant, but avoids the undesirable result of the migration of the transgene into related host systems and/or to the environment via the pollen. The methods described herein may also be applied to the expression of virtually any protein of interest (e.g. a toxic protein) in eukaryotic (yeast, insect, mammalian cells, etc.) and prokaryotic (<i>E. coli</i>, etc.) organisms.
L'invention concerne un nouveau système transgénique qui permet l'expression protéique efficace dans un hôte cible tel qu'une plante, tout en évitant la migration indésirable du transgère dans les systèmes hôtes associés et/ou dans l'environnement par le pollen. Les procédés décrits ici peuvent être appliqués à l'expression de quasiment toute protéine d'intérêt (p. ex. une protéine toxique) dans les organismes eucaryotes (levure, insecte, cellules mammifères, etc.) et dans les organismes procaryotes (<i>E. coli</i>, etc.).
Chen Lixin
Comb Donald G.
Evans Thomas C.
Ghosh Inca
Paulus Henry
Boston Biomedical Research Institute
New England Biolabs Inc.
Ridout & Maybee Llp
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