Method for generating variable domain sequences of heavy...

C - Chemistry – Metallurgy – 12 – N

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Details

C12N 15/13 (2006.01) C07K 16/00 (2006.01) C12N 15/09 (2006.01) C12N 15/63 (2006.01) C12P 21/08 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2595682

The present invention relates to a method for generating or cloning a nucleic acid or nucleotide sequence that encodes a heavy chain antibody or an antigen- binding fragment thereof, wherein said heavy chain antibody or antigen-binding fragment is directed against a specific antigen, said method comprising the steps of providing a sample or population of cells from a Camelid immunized with said antigen, isolating from said sample or population said at least one cell that expresses or is capable of expressing a heavy chain antibody directed against said antigen, and obtaining from said at least one cell a nucleic acid or nucleotide sequence that encodes a heavy chain antibody directed against antigen or that encodes an antigen-binding fragment thereof directed against said antigen.

La présente invention porte sur un procédé permettant de générer ou de cloner une séquence d'acides nucléiques ou de nucléotides qui code un anticorps à chaîne lourde ou un fragment de liaison à l'antigène de celui-ci, lequel anticorps à chaîne lourde ou le fragment de liaison à l'antigène de celui-ci est dirigé contre un antigène spécifique. Le procédé de cette invention comprend les étapes consistant: à utiliser un échantillon ou une population de cellules d'un camélidé immunisé avec cet antigène; à isoler de cet échantillon ou de cette population la ou les cellules qui expriment ou qui sont capables d'exprimer un anticorps à chaîne lourde dirigé contre cet antigène; et à obtenir à partir de la ou des cellules une séquence d'acides nucléiques ou de nucléotides qui code un anticorps à chaîne lourde dirigé contre un antigène ou qui code un fragment de liaison à l'antigène dirigé contre ledit antigène.

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