Method for generation of immunoglobulin sequences

A - Human Necessities – 61 – K

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A61K 39/00 (2006.01) C07K 16/28 (2006.01)

Patent

CA 2747644

The present invention relates to a method for generating immunoglobulin sequences against cell -associated antigens, more particularly, antigens that are membrane- anchored. The invention also provides immunoglobulin sequences obtainable by the method of the invention. Specifically, the present invention relates to the generation of immunoglobulin sequences by use of DNA vaccination. More specifically, the present invention relates to generation of immunoglobulin sequences in camelids, preferably directed against cell-associated antigens, in particular antigens with multiple transmembrane spanning domains, including GPCRs and ion channels, by DNA vaccination. Furthermore, the present invention relates to said immunoglobulin sequences against cell-associated antigens, more particularly, antigens that are membrane-anchored, such as e.g. GPCRs and ion channels, more preferably ion channels.

Cette invention concerne un procédé de génération de séquences d'immunoglobulines dirigées contre des antigènes associés à des cellules, plus particulièrement des antigènes ancrés à la membrane. L'invention concerne également les séquences d'immunoglobulines qui peuvent être obtenues par le procédé selon l'invention. Plus spécifiquement, la présente invention concerne la génération de séquences d'immunoglobulines par l'intermédiaire de la vaccination par ADN. Plus spécifiquement encore, la présente invention concerne la génération de séquences d'immunoglobulines chez les camélidés, dirigées de préférence contre des antigènes associés à des cellules, en particulier les antigènes à multiples domaines transmembranaires, notamment les GPCR et les canaux ioniques, par la vaccination par ADN. En outre, la présente invention concerne lesdites séquences d'immunoglobulines dirigées contre les antigènes associés à des cellules, plus particulièrement les antigènes qui sont ancrés à la membrane, notamment les GPCR et les canaux ioniques, de préférence les canaux ioniques.

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