Method for genome mining for secreted protein genes

C - Chemistry – Metallurgy – 12 – N

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C12N 15/10 (2006.01) C12N 9/00 (2006.01)

Patent

CA 2412502

The present invention relates to a method for screening for compounds secreted by an organism, comprising (a) Raising antibodies against secreted products of a donor organism, (b) providing a gene library from the donor organism, (c) cloning the gene library into a suitable host organism, (d) expressing the cloned genes in the host organism, and (e) detecting positive clones, which upon expression of the cloned genes secretes a compound, using the antibodies of (a) to identify such positive clones. The invention also relates to compounds, nucleotide sequences and microorganisms identifiable by said method.

La présente invention concerne une méthode de criblage de composés sécrétés par un organisme, qui consiste à: a) activer des anticorps contre des produits sécrétés d'un organisme donneur; b) mettre en oeuvre une bibliothèque de gènes provenant de l'organisme donneur; c) cloner la bibliothèque de gènes pour obtenir un organisme hôte approprié; d) exprimer les gènes clonés dans l'organisme hôte; et e) détecter des clones positifs sécrétant un composé après expression des gènes clonés, et utiliser les anticorps évoqués en a) pour identifier lesdits clones positifs. L'invention concerne en outre des composés, des séquences nucléotidiques et des micro-organismes pouvant être identifiés par la méthode de l'invention.

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