Method for genotyping microsatellite dna markers

Details Method for genotyping microsatellite dna markers Method for genotyping microsatellite dna markers

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2445095

The present invention provides a high throughput method for genotyping microsatellite markers. The technology uses a combination of oligonucleotides in a selective ligation reaction that discriminates mono-, di-, tri,- tetra-, penta-, hexa-, hepta-, octa- and nona-nucleotide repeated alleles in amplified DNA from individuals.

L'invention concerne une méthode de génotypage à haut débit de marqueurs microsatellites. Cette technique fait intervenir une combinaison d'oligonucléotides dans une réaction de ligature sélective qui différencie des allèles répétés mono-, di-, tri,- tétra-, penta-, hexa-, hepta-, octa- et nona-nucléotidiques dans un ADN amplifié issu de personnes.

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