Method for growth and purification of human cell lines from...

Details Method for growth and purification of human cell lines from... Method for growth and purification of human cell lines from...

C - Chemistry, Metallurgy

12

N

167/129, 195/46,

C12N 5/02 (2006.01) A61K 39/395 (2006.01) C12N 5/06 (2006.01) G01N 33/50 (2006.01) G01N 33/569 (2006.01) G01N 33/574 (2006.01) G01N 33/577 (2006.01)

Patent

CA 1233135

SK 284 ABSTRACT Human melanocyte cells are grown in tissue culture medium containing fibroblast growth suppressor plus tumor growth promoters at pH 7.2 to about 7.4. In a stepwise procedure, Langerhans, keratinocytes and most fibroblasts are removed in the presence of the above suppressor and promoter followed by a subsequent trypsinization treatment. In a final purification step, remaining fibroblasts are separated from the melanocytes by reacting the cell mixture with monoclonal antibody specific for melanocytes, and then separating out the melanocytes from the fibroblasts on density gradients.

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