Method for highly purifying human serum albumin

Details Method for highly purifying human serum albumin Method for highly purifying human serum albumin

C - Chemistry, Metallurgy

07

K

C07K 14/765 (2006.01) C07K 1/36 (2006.01) C12N 15/14 (2006.01) C12N 15/81 (2006.01)

Patent

CA 2141439

A method of highly purifying human serum albumin (HSA) which comprises bringing a fraction containing HSA pro- duced by gene manipulation into contact with a chelate chromatography carrier having copper ions combined therewith and elut- ing the HSA adsorbed on the carrier by using a buffer having a pH of 5 to 7 and containing ammonium chloride as an antagonist. This method can provide a highly pure HSA free from yeast-origin components which have been dificult to remove satisfactorily by the conventional method of purifying HSA produced by gene manipulation.

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