C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01) C07H 21/04 (2006.01)
Patent
CA 2098947
- 59 - RAN 4095/26-30 Abstract A process for determining an individual's HLA DP genotype from a nucleic acid containing sample obtained from the individual, which process comprises: (a) amplifying a target region of the nucleic acids in the sample under conditions suitable for carrying out a polymerase chain reaction, whereby the target region contains a polymorphic region (variable segment) of an HLA DP gene using a specific primer; (b) mixing the amplified nucleic acids with a panel of sequence specific oligonuclcotide (SSO) probes, wherein each probe is complementary to a variant sequence of a variable segment of an HLA DP gene, under conditions wherein SSO probes bind to said amplified nucleic acids to form stable hybrid duplexes only if they are exactly complementary; and (c) detecting hybrids formed between the amplified nucleic acids and the SSO probes. The invention also relates to the said primers per se, to kits comprising said oligonuclcotide primers and to ligonuclcotide probes useful in HLA-DP DNA typing methods. These HLA- DP DNA typing methods may be useful in the prevention of graft rejection and graft versus host disease, in determining susceptibility to autoimmune diseases, in providing evidence concerning the derivation from an individual of forensic samples, and in paternity testing.
Begovich Ann B.
Bugawan Teodorica L.
Erlich Henry A.
Borden Ladner Gervais Llp
F. Hoffmann-La Roche Ag
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