Method for identification of tr1 lymphocytes regulators by...

A - Human Necessities – 61 – K

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A61K 39/00 (2006.01) C12Q 1/02 (2006.01) C12Q 1/68 (2006.01) G01N 33/50 (2006.01) G01N 33/68 (2006.01) C12N 5/078 (2010.01)

Patent

CA 2529941

The invention relates to a method for identification of Tr1 lymphocyte regulators in a biological sample, based on the determination of the simultaneous presence of the molecular group CD4, CD18 and/or CD11a, CD49b and, where appropriate, by the demonstration of an over-expression of genes coding for the molecules CD4, PSGL-1, PECAM-1 and alphaV/beta3. The invention further relates to a method of quantification and a prognostic or diagnostic method for auto-immune or inflammatory diseases, based on said identification method. The invention also relates to an enrichment method for Tr1 lymphocyte regulators, based on the determination of the simultaneous presence of said molecules and, finally, an enriched composition from said enrichment method, for the treatment of an auto-immune or inflammatory disease, in particular Crohn's disease.

L'invention a pour objet un procédé d'identification de lymphocytes Tr1 régulateurs dans un échantillon biologique basé sur la détermination de la présence simultanée du groupe de molécules CD4, CD18 et/ou CD11a, CD49b et, le cas échéant, par la mise en évidence d'une surexpression des gènes codant pour les molécules CD4, PSGL-1, PECAM-1 et alphaV/beta3. L'invention a également pour objet un procédé de quantification et un procédé de pronostic ou de diagnostic de maladies auto-immunes ou inflammatoires basé sur ledit procédé d'identification. L'invention a également pour objet un procédé d'enrichissement en lymphocytes Tr1 régulateurs basé sur la détermination de la présence simultanée de ces molécules. L'invention a enfin pour objet l'utilisation d'une composition enrichie selon ledit procédé d'enrichissement pour le traitement d'une maladie auto-immune ou inflammatoire, notamment la maladie de Crohn.

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