Method for identification of type specific polynucleotide...

Details Method for identification of type specific polynucleotide... Method for identification of type specific polynucleotide...

C - Chemistry, Metallurgy

12

Q

C12Q 1/70 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2456568

A process for identification of type-specific polynucleotide sequences in a sample, the process comprising the steps of (l) contacting polynucleotides from the sample, or derived from the sample, with a plurality of type-specific probes in the context of a solid support, and detection of any type-specific hybridisation; and (2) contacting polynucleotides in the sample, or derived from the sample, with type-specific primers for those types capable of being detected by the hybridisation step in step 1, but not so detected, in a type- specific amplification reaction.

L'invention concerne un procédé d'identification de séquences polynucléotidiques de type spécifique dans un échantillon, ledit procédé consistant (1) à mettre en contact les polynucléotides de l'échantillon, ou dérivés de l'échantillon, avec une pluralité de sondes de type spécifique dans le contexte d'un support solide et de la détection d'une hybridation de n'importe quel type spécifique; et (2) à mettre en contact les polynucléotides de l'échantillon, ou dérivés de l'échantillon, avec des sondes de type spécifique pour les types pouvant être détectés grâce à l'hybridation de l'étape 1, mais non détectés dans une réaction d'amplification de type spécifique.

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