C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01)
Patent
CA 2413423
The invention relates to a new method for sequence-specific identification, separation and quantitative measurement of nucleic acid fragments. The invention is based on the use of restriction endonucleases that have degenerate bases in their recognition or cleavage sequence. The method has broad applications, including DNA fingerprinting, differential display of mRNA, mutation and polymorphism identification, diagnosis and drug screening.
L'invention concerne un nouveau procédé d'identification, de séparation et de mesure quantitative, spécifique à une séquence, de fragments d'acide nucléique. L'invention repose sur l'utilisation d'endonucléases de restriction comprenant des bases dégénérées dans leur site de reconnaissance ou de restriction. Ce procédé possède un grand champ d'application, y compris dans la technique des empreintes génétiques, dans l'affichage différentiel de l'ARNm, dans l'identification des mutations et des polymorphismes, dans les diagnostiques et le criblage de médicaments.
Li Bi-Yu
Shi Liang
Wang Xun
Fetherstonhaugh & Co.
Syngenta Participations Ag
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