Method for identifying a presenilinase inhibitor

G - Physics – 01 – N

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Details

G01N 33/68 (2006.01) A01K 67/027 (2006.01) C07K 14/47 (2006.01) G01N 33/573 (2006.01)

Patent

CA 2332344

The present invention pertains to methods for identifying a substance capable of reducing or eliminating the activity of the presenilinase wherein a cell or a cell line is cultivated expressing said presenilinase activity and a fusion- protein comprising the full-length presenilin 1 or presenilin 2 and a reporter is measured. The invention is furthermore concerned with substances identifiable with said methods, pharmaceutical compositions comprising said substances and the use of said substances in the manufacture of a medicament for the treatment of neurodegenerative diseases or Alzheimer's disease.

L'invention concerne des méthodes d'identification d'une substance capable de réduire ou de supprimer l'activité de la présénilinase. On cultive une cellule ou une ligné cellulaire exprimant ladite activité de la présénilinase et on mesure une protéine de fusion contenant la préséniline 1 ou la préséniline 2 pleine longueur et un rapporteur. L'invention concerne également des substances identifiables grâce aux méthodes selon l'invention, des compositions pharmaceutiques contenant ces substances et l'utilisation de ces substances dans la fabrication d'un médicament pour le traitement des maladies neurodégénératives, notamment de la maladie d'Alzheimer.

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