Method for identifying compounds affecting rna/rna binding...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) C07C 281/18 (2006.01) C07D 471/06 (2006.01) C12N 15/10 (2006.01) C40B 30/04 (2006.01) G01N 33/53 (2006.01)

Patent

CA 2591585

It has been discovered that a single set of conditions can be used to detect nearly every interaction of RNA binding proteins and RNA molecules. Prior to this discovery it was thought that each specific interaction required separate optimized conditions in order to be detected. Assays employing the disclosed universal conditions are useful for identifying RNA binding proteins that interact with specific RNA molecules of interest, detecting RNA molecules that interact with specific RNA binding proteins of interest, identifying RNA binding proteins active in certain cell types and under certain physiological conditions, identifying specific regions of an RNA molecule that interact with RNA binding proteins. Screening assays employing the disclosed universal conditions are useful for identifying compounds that modulate RNA/RBP interactions of interest.

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