C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/04 (2006.01) C12M 1/34 (2006.01) G01N 33/68 (2006.01) G06F 17/00 (2006.01) G06F 19/00 (2011.01)
Patent
CA 2751693
This invention relates to a method for identifying germs in a liquid medium, comprising adding a membrane detergent to the liquid medium containing components of a host infected with germs, i.e. cellular components and proteins from the extracellular environment, so as to release the germs from these components without degrading them, and in that the germs that have grown in the liquid medium are analysed by MALDI-TOF MS.
Cette invention concerne un procédé permettant d'identifier des germes dans un milieu liquide, ledit procédé consistant à ajouter un détergent membranaire au milieu liquide contenant les composants d'un hôte infecté par les germes (composants cellulaires et protéines de l'environnement extracellulaire), de manière à libérer les germes de ces composants sans les dégrader ; les germes qui se sont multipliés dans le milieu liquide sont analysés par spectrométrie de masse au moyen de l'instrument MALDI-TOF.
Beretti Jean-Luc
Carbonnelle Etienne
Dauphin Brunhilde
Ferroni Agnes
Nassif Xavier
Assistance Publique-Hopitaux de Paris
Benoit & Cote Associes Inc.
Universite Paris Descartes
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