Method for identifying human subjects by analysis of genetic...

Details Method for identifying human subjects by analysis of genetic... Method for identifying human subjects by analysis of genetic...

C - Chemistry, Metallurgy

07

H

195/1.12, 150/8.

C07H 21/00 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2013430

ABSTRACT OF THE DISCLOSURE A method for genetic typing of human DNA is disclosed. The method has utility in detecting microsatellite polymorphisms in DNA from a sample of human individuals selected from a population of individuals and in determining a human subject from a sample of biological material containing human DNA. The method examines polymorphisms of a (dT-dG)n VNTR region at the D11S35 locus on human chromosome 11. The method comprises the steps of isolating the human DNA, heat-denaturing the DNA to form single strands, hybridizing synthetic DNA oligomers as described herein to the denatured DNA so as to generate primer sites adjacent said (dT-dG)n regions on the DNA, amplifying the D11S35 VNTR region using the polymerase chain reaction method, and separating the amplified segments. Different individuals exhibit distinctive allelic patterns of separated segments, which can be used to confirm identities, pedigrees, etc., and in forensic studies. The sequences of preferred synthetic DNA oligomer primers, termed oligomers 780 and 781, have been determined and are disclosed herein.

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