Method for identifying individual active entities from...

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/00 (2006.01) C12Q 1/68 (2006.01) C12Q 1/70 (2006.01) G01N 33/53 (2006.01) G01N 33/566 (2006.01) G01N 33/567 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2491969

A method of screening a mixture for active entities, which method comprises: providing a plurality of ligands, wherein each ligand is attached to a support to form a plurality of ligand-support complexes, contacting the ligand-support complexes with a mixture comprising a plurality of entities under conditions that allow at least one entity to bind to at least one ligand-support complex, thereby forming at least one entity-ligandsupport complex, separating at least one entity-ligand-support complex from the unbound entities, assaying at least one entity of at least one entity-ligand~support- complex for an activity, detecting the activity, and selecting at least one entity-ligand-support- complex having the entity, which exhibited the detected activity, whereupon a mixture is screened for active entities; and related methods.

L'invention concerne un procédé permettant le criblage d'un mélange en vue de séparer des entités actives. Ce procédé consiste à préparer une pluralité de ligands, chaque ligand étant relié à un support, de manière à former une pluralité de complexes ligand- support, a faire entrer en contact ces complexes ligand-support avec un mélange contenant une pluralité d'entités dans des conditions permettant la liaison d'au moins une entité avec au moins un complexe ligand-support, ce qui entraîne la formation d'au moins un complexe entité-ligand-support, à séparer au moins un complexe entité-ligand-support des entités libres, à analyser au moins une entité d'au moins un complexe entité-ligand-support afin d'identifier son activité, et à sélectionner au moins un complexe entité-ligand-support qui comprend l'entité présentant l'activité détectée, le mélange étant alors soumis à un criblage afin de permettre l'identification des entités actives, ainsi que des procédés associés.

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