Method for identifying ligands specific for structural...

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G01N 33/543 (2006.01) G01N 33/53 (2006.01) G01N 33/551 (2006.01) G01N 33/566 (2006.01)

Patent

CA 2522483

A method of identifying a ligand specific for a structural isoform of a protein by the binding of the structural isoform to a ligand on a support, a direct positional transfer of the structural isoform and a control isoform between two or more different solid or semi-solid supports and detection of at least one of the isoforms on each support, thus allowing for subtractive identification techniques to be used to identify the subset of ligands, or a ligand, specific for the structural isoform (Figure 1).

L'invention porte sur un procédé d'identification d'un ligand spécifique pour une isoforme structurale d'une protéine par liaison de l'isoforme structurale à un ligand sur un support, par transfert de position direct de l'isoforme structurale et d'une isoforme de contrôle entre au moins deux supports différents solides ou semi-solides et la détection d'au moins une des isoformes sur chaque support, ce qui permet d'utiliser des techniques d'identification soustractives pour identifier le sous-ensemble de ligands, ou un ligand, spécifique de l'isoforme structurale.

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