C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01) C12N 15/11 (2006.01)
Patent
CA 2363938
The present invention relates to a method of identifying a microorganism in a sample, based upon sequencing, and analysing, using a sequencing-by- synthesis procedure, short stretches, or fragments of a gene. Accordingly, the present invention provides a method of identifying a microorganism in a sample, said method comprising: determining the sequence of a region of up to 50 nucleotides in a predetermined site in a gene of said microorganism, thereby to obtain a signature sequence; and analysing sequencing information in said signature sequence to identify said microorganism, wherein said sequence is determined by detecting the nucleotides incorporated in a primer extension reaction performed using a primer binding at a pre- determined site in said gene.
Fetherstonhaugh & Co.
Pyrosequencing Ab
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