Method for identifying modulators of the activity of...

G - Physics – 01 – N

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G01N 33/68 (2006.01) G01N 33/566 (2006.01)

Patent

CA 2590322

The present invention is directed to a method for selecting or identifying a compound capable of modulating the activity of an ion-channels receptor, such as GABA receptor, by measuring the calcium influx via the P2X receptor in presence of its agonist ATP when coupled to said ion-channels receptor in recombinant cells co~expressing these two receptors. The invention also concerns a kit or a device comprising the essentiel elements to perform the method according to the invention. Finally, the present invention is directed to the use of said selected compounds for the prevention or the treatment of diseases related to said ion-channels receptor dysfunction.

L'invention concerne un procédé de sélection ou d'identification d'un composé capable de moduler l'activité d'un récepteur à canaux ioniques, tel qu'un récepteur GABA, procédé consistant à mesurer l'influx de calcium via le récepteur P2X en présence de son agoniste ATP lorsqu'il est couplé audit récepteur à canaux ioniques dans des cellules recombinantes co-exprimant ces deux récepteurs. L'invention concerne également un kit ou un dispositif comprenant les éléments essentiels pour la mise en oeuvre du procédé de l'invention. Enfin, l'invention concerne l'utilisation desdits composés sélectionnés, pour la prévention ou le traitement de maladies associées à un dysfonctionnement dudit récepteur à canaux ioniques.

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