Method for identifying molecular weight of a phosphoric acid...

Details Method for identifying molecular weight of a phosphoric acid... Method for identifying molecular weight of a phosphoric acid...

G - Physics

01

N

G01N 33/483 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2518495

The present invention provides a method for confirming existence of a phosphoric acid monoester compound (peptide, saccharine and the like) and easily identifying the molecular weight thereof even among biological samples including a plurality of compounds, and an additive for a mass spectrometry used for the method. In the method according to the present invention, a complex compound exhibiting extremely high coordination ability to a phosphoric acid monoester group and configured of single kind of zinc isotopes is used to obtain a plurality of mass spectrum data, and then the data are compared.

L'invention concerne un procédé permettant, dans un échantillon biologique contenant de nombreux composants, non seulement de confirmer la présence d'un composé converti en monoester d'acide phosphorique (peptide, sucre, etc.) parmi ces composants, mais également d'identifier facilement son poids moléculaire. L'invention concerne également un additif pour une mesure à spectre de masse qui peut être utilisé pour ledit procédé. Dans le procédé de l'invention, un composé complexe, présentant une capacité de coordination extrêmement élevée pour des groupes monoesters d'acide phosphorique, est utilisé et il comprend un isotope de zinc simple. Les données à spectre de masse multiple sont collectées et comparées les unes avec les autres.

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