Method for identifying substances useful for treating...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) A61K 31/00 (2006.01) A61P 29/00 (2006.01) C12N 5/10 (2006.01) C12N 15/63 (2006.01) C12N 15/85 (2006.01) G01N 33/566 (2006.01)

Patent

CA 2420233

The invention concerns a method for identifying substances useful for treating inflammation, characterised in that it comprises the following steps: (a) contacting said substance with a nucleic acid construct containing the response element to the ROR receptor of the promoter of the I.kappa.B.alpha. gene in conditions suitable for allowing said substance to be fixed on the response element; (b) measuring the possible fixing of said substance on the response element or the transcriptional activity of the response element or of a promoter containing it; (c) optionally comparing the measurement of step (b) with a measurement carried out in conditions similar to those of steps (a) and (b) but with a nucleic acid construct non containing the mutated response element.

La présente invention concerne un procédé d'dentification de substances utiles pour le traitement de l'inflammation, caractérisé en ce qu'il comprend les étapes suivantes: (a) la mise en contact de ladite substance avec une construction d'acide nucléique comprenant l'élément de réponse au récepteur ROR du promoteur du gène I.kappa.B.alpha., dans des conditions susceptibles de permettre à ladite substance de se fixer sur ledit élément de réponse, (b) la mesure de la fixation éventuelle de ladite substance sur l'élément de réponse, (b) la mesure de la fixation éventuelle de ladite substance sur l'élément de réponse ou de l'activité transcriptionelle de l'élément de réponse ou d'un promoteur le contenant, (c) éventuellement la comparaison de la mesure de l'étape (b) avec une mesure réalisée dans les mêmes conditions qu'aux étapes (a) et (b) mais avec une construction d'acide nucléique comprenant l'élément de réponse muté.

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