Method for identifying target protein of agent and method...

G - Physics – 01 – N

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G01N 33/53 (2006.01) G01N 33/15 (2006.01) G01N 33/50 (2006.01) G01N 33/566 (2006.01) C07K 14/47 (2006.01) C12N 15/09 (2006.01)

Patent

CA 2601869

A method for identifying a target protein of a compound having a pharmacological action by detecting a tertiary structural change of a target protein by binding a compound having a pharmacological action to a target protein with the use of a molecular chaperone protein having a characteristic of binding to a protein by recognizing a tertiary structural change of the protein is disclosed. Further, a method for screening a therapeutic agent for diabetes using a target protein of biguanide which is a therapeutic agent for diabetes and was found by the identification method, a screening tool which can be used in the screening method and a pharmaceutical composition for treating diabetes containing a substance obtained by the screening method are disclosed.

Il est exposé un procédé servant à identifier une protéine cible d'un composé ayant une action pharmacologique en détectant une modification de la structure tertiaire d'une protéine cible produite par la formation d'une liaison du composé ayant une action pharmacologique à cette protéine cible, grâce à l'utilisation d'une protéine chaperon moléculaire ayant la caractéristique de se lier à une protéine en reconnaissant une modification de la structure tertiaire de cette protéine. En outre, il est exposé un procédé servant à cribler un agent thérapeutique pour le diabète utilisant une protéine cible du biguanide, lequel est un agent thérapeutique pour le diabète, qui a été trouvée par le procédé d'identification ; un outil de criblage qu'on peut utiliser dans le procédé de criblage ; et une composition pharmaceutique servant à traiter le diabète contenant une substance obtenue par le procédé de criblage.

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