Method for identifying the ligands of a receptor capable of...

G - Physics – 01 – N

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G01N 33/50 (2006.01) G01N 33/566 (2006.01) G01N 33/58 (2006.01)

Patent

CA 2359213

The invention concerns a method useful for detecting and/or identifying in a bank of peptides, pseudopeptides and non-peptide compounds, a biological extract or a purified fraction of a tissue extract, a ligand of a receptor of interest capable of being subjected to internalization induced by immobilising said ligand. The invention is characterised in that it comprises at least steps which consist in: expressing at the surface of a cell said receptor in a marked form; bringing in contact said cell and said bank in conditions suitable for cell internalization of said ligand-receptor complex; and displaying said internalization via the detection of the marker associated with said receptor.

L'invention a pour objet un procédé utile pour détecter et/ou identifier dans une banque de composés peptidiques, pseudopeptidiques ou non-peptidiques, un extrait biologique ou une fraction purifiée d'un extrait de tissus, un ligand d'un récepteur d'intérêt et capable de subir une internalisation induite par la fixation dudit ligand caractérisé en ce qu'il comprend au moins les étapes consistant à: exprimer à la surface d'une cellule ledit récepteur sous une forme marquée; mettre en présence ladite cellule avec ladite banque dans des conditions suffisantes pour permettre une internalisation cellulaire dudit complexe récepteur-ligand et visualiser cette internalisation via la détection du marqueur associé audit récepteur.

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