C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
530/17.08
C12N 11/08 (2006.01)
Patent
CA 1087608
ABSTRACT OF THE DISCLOSURE Biologically-active proteins are immobilized by a method in which a compound containing at least two secondary amino groups is reacted at a temperature of from -80 to +50°C. with a chlorination agent with the conversion of the secondary amino groups into imino-chloride groups of the general formula:- R - ? = N - R1 in which R and R1, which can be the same or different, are ali- phatic, aromatic or aliphatic-aromatic radicals, and the product thus obtained is either reacted in an aqueous solvent directly with a biologically-active protein, or is reacted with a bifunc- tional compound containing at least one primary amino group, optionally in an aqueous or organic solvent, and the product thereby obtained with groups of the general formula:- Image in which R2 is an alkylene radical, an .alpha., .omega.-dioxyalkylene radical or a dicarboxylic acid amide group containing 2 to 8 carbon atoms and X is a primary amino group or a function capable of protein binding, is either reacted directly with a protein or is first reacted with at least one bifunctional or polyfunctional pro- tein reagent and thereafter reacted with a protein or with a reac- tion product of a protein with the protein reagent, the immobil- ization of biologically active proteins is useful in preparation and analytical chemistry.
267721
Batz Hans-Georg
Horn Jurgen
Jaworek Dieter
Boehringer Mannheim G.m.b.h.
Sherman
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