G - Physics – 01 – N
Patent
G - Physics
01
N
167/44
G01N 33/566 (2006.01) G01N 33/53 (2006.01) G01N 33/543 (2006.01)
Patent
CA 2027035
ABSTRACT OF THE DISCLOSURE: A method for immunochemical determination of a hapten in a sample is disclosed, in which (A) a high-molecular compound to which the hapten is bound (reagent A), (B) insoluble carrier particles carrying thereon an antibody to the hapten (reagent B), and (C) magnetic substance-containing insoluble carrier particles carrying thereon an antibody to an antigenic determinant in the high-molecular compound and different from the hapten (reagent C), are used. These three reagents are dispersed in the sample, then, a magnetic field is applied to separate from the reaction mixture unreacted reagent (C) and agglutinated particles formed from the reagent (B) and the reagent (C) through the reagent (A). The amount of the reagent (B) remaining dispersed in the reaction mixture is measured, thereby determining the extent of competitive inhibition to the agglutination of the reagent (B) and the reagent (C) through the reagent (A) by the reaction between the hapten in the sample and the reagent (B). The method of the present invention requires no troublesome operations such as separation- washing and is free from any problem in safety. By the method of the present invention, the concentration of a hapten in a sample is determined by simple operations in a short time and with high sensitivity. - 27 -
Ito Michio
Kohno Hideki
Ogura Minoru
Mitsubishi Chemical Corporation
Ridout & Maybee Llp
LandOfFree
Method for immunochemical determination of hapten does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for immunochemical determination of hapten, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for immunochemical determination of hapten will most certainly appreciate the feedback.
Profile ID: LFCA-PAI-O-1900766