C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/11 (2006.01) C12N 15/64 (2006.01)
Patent
CA 2588936
The present invention is directed to a method for inactivation of expression of a gene in a eucaryotic cell comprising (I) transfection of a eucaryotic cell with DNA comprising an expression cassette for expression of a cell surface protein and an expression cassette for expression of a RNAi compound, said compound being capable of inactivating expression of said gene, wherein said expression cassette for expression of a cell surface protein and said expression cassette for expression of a RNAi compound are located on the same vector DNA, and (II) enrichment and/or selection of cells which express said cell surface protein.
La présente invention concerne une méthode d'inactivation de l'expression d'un gène dans une cellule eucaryote. Ladite méthode comprend (I) la transfection d'une cellule eucaryote par un ADN comprenant une cassette d~expression permettant l'expression d'une protéine de surface cellulaire, et une cassette d'expression permettant l'expression d'un composé de type ARNi, ledit composé de type ARNi étant susceptible d'inactiver l'expression dudit gène, et lesdites cassettes d'expression étant situées sur le même ADN vecteur, et (II) la sélection de et/ou l'enrichissement en cellules qui expriment ladite protéine de surface cellulaire.
Kubbies Manfred
Macek Robert
Ries Carola
F. Hoffmann-La Roche Ag
Gowling Lafleur Henderson Llp
LandOfFree
Method for improved selection of rnai transfectants does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for improved selection of rnai transfectants, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for improved selection of rnai transfectants will most certainly appreciate the feedback.
Profile ID: LFCA-PAI-O-1890402