Method for improving quantitative recovery of microorganisms...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/06 (2006.01) C12N 1/38 (2006.01) C12Q 1/04 (2006.01) C12Q 1/22 (2006.01)

Patent

CA 2176810

A method for increasing the quantitation of microorganisms in cell-containing or cell-free blood samples, included those intended for blood product transfusion purposes, is provided which employs a sustained level of sodium polyanethol sulfonate throughout microbial replication on solid media. In another embodiment, purified saponin is combined with sodium polyanethol sulfonate to provide increased quantitation of microorganisms.

Procédé permettant d'améliorer l'analyse quantitative de micro-organismes dans des échantillons sanguins contenant des cellules ou qui en sont dépourvus, y compris ceux destinés à la transfusion de produits sanguins. Ce procédé consiste à utiliser un niveau constant de sulfonate de polyéthanol de sodium au cours de toute la réplication microbienne sur un milieu solide. Dans un autre mode de réalisation, de la saponine purifiée est combinée avec du sulfonate de polyéthanol de sodium afin de permettre une analyse quantitative améliorée de micro-organismes.

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