Method for improving stability and shelf-life of liposome...

A - Human Necessities – 61 – K

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A61K 9/127 (2006.01)

Patent

CA 2528411

A method for preparing a stable cell-targeting complex comprising a ligand and a cationic liposome encapsulating a therapeutic or diagnostic agent comprises (a) combining the complex with a solution comprising a stabilizing amount of sucrose and (b) lyophilizing the resultant solution to obtain a lyophilized preparation; wherein, upon reconstitution, the preparation retains at least about 80% of its pre-lyophilization activity.

L'invention concerne un procédé de préparation d'un complexe stable de ciblage de cellules, comprenant un ligand et un liposome cationique encapsulant un agent thérapeutique ou diagnostique. Ladite méthode consiste (a) à combiner le complexe à une solution comprenant une quantité stabilisante de saccharose et (b) à lyophiliser la solution résultante pour obtenir une préparation lyophilisée. Lors de la reconstitution, la préparation conserve au moins environ 80 % de son activité de pré-lyophilisation.

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