Method for improving stress tolerance during fermentation

Details Method for improving stress tolerance during fermentation Method for improving stress tolerance during fermentation

C - Chemistry, Metallurgy

12

N

C12N 1/21 (2006.01) C12N 1/15 (2006.01) C12N 1/19 (2006.01) C12N 5/10 (2006.01) C12N 15/53 (2006.01) C12N 15/61 (2006.01) C12N 15/63 (2006.01) C12P 7/56 (2006.01)

Patent

CA 2561408

A method of increasing stress tolerance in recombinant organisms that have been engineered for industrial production is described. Stress tolerance is increased by making L-ascorbic acid available to the recombinant organism, either by exogenous addition to the culture medium or by endogenous production from D-glucose by the recombinant organism. To enable endogenous production, the recombinant organism is transformed with a coding region encoding a mannose epimerase (ME), a coding region encoding an L-galactose dehydrogenase (LGDH), and a D-arabinono-1,4-lactone oxidase (ALO). The recombinant organism may be further transformed with myoinositol phosphatase (MIP/VTC4).

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