Method for improving the agronomic and nutritional value of...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/52 (2006.01) A01H 5/00 (2006.01) C12N 5/10 (2006.01) C12N 9/00 (2006.01) C12N 9/02 (2006.01) C12N 15/53 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2362448

The present invention provides means and methods of transforming plant cells, seeds, tissues or whole plants in order to yield transformants capable of expressing all enzymes of the carotenoid biosynthesis pathway that are essential for the targeted host plant to accumulate carotenes and/or xanthophylls of interest. The present invention also provides DNA molecules designed to be suitable for carrying out the method of the invention, and plasmids or vector systems comprising said molecules. Furthermore, the present invention provides transgenic plant cells, seeds, tissues and whole plants that display an improved nutritional quality and contain such DNA molecules and/or that have been generated by use of the methods of the present invention.

La présente invention concerne des moyens et des procédés de transformation de cellules de plantes, de semences de tissus ou de plantes entières afin d'obtenir des transformants capables d'exprimer toutes les enzymes de la voie de biosynthèse des caroténoïdes qui sont essentiels pour que la plante hôte ciblée puisse accumuler des carotènes et/ou des xanthophylles recherchés. L'invention concerne aussi des molécules d'ADN conçues afin de mettre en oeuvre le procédé de l'invention, ainsi que des plasmides et des vecteurs contenant ces molécules. La présente invention concerne, en outre, des cellules de plantes transgéniques, des semences, des tissus et des plantes entières qui possèdent une qualité nutritionnelle améliorée et qui contiennent de telles molécules d'ADN et/ou qui ont été générées au moyen des procédés de la présente invention.

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