Method for improving the performance of an immunoreagent in...

G - Physics – 01 – N

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G01N 33/543 (2006.01) G01N 33/545 (2006.01)

Patent

CA 2255909

A method for increasing the binding activity of specific binding members bound to a solid phase material, e.g., a particle, that has been sterically stabilized. This increase in binding activity is brought about by degrading a steric stabilizer on the surface of the solid phase material. The method involves both immobilizing a specific binding member on the surface of a solid phase material and degrading a steric stabilizer on the surface of that solid phase material. In the preferred embodiment, the method involves the immobilization of a specific binding member on the surface of the sterically stabilized solid phase material, with subsequent degradation of the steric stabilizer.

Procédé permettant d'accroître l'activité de liaison d'éléments de liaison spécifiques liés à une substance en phase solide, telle qu'une particule, qui a été stériquement stabilisée. Cet accroissement de l'activité de liaison résulte de la dégradation d'un stabilisant stérique à la surface de la substance en phase solide. Le procédé implique l'immobilisation d'un élément de liaison spécifique à la surface de la substance en phase solide et la dégradation d'un stabilisant stérique à la surface de ladite substance en phase solide. Dans la forme de réalisation préférée, le procédé consiste à immobiliser un élément de liaison spécifique à la surface de la substance en phase solide stériquement stabilisée, puis à laisser se dégrader le stabilisant stérique.

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