C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 7/00 (2006.01) A61K 39/12 (2006.01) C12N 5/10 (2006.01) G01N 33/569 (2006.01) C12N 5/06 (2006.01)
Patent
CA 2380407
The invention concerns a method of culturing, propagating and replicating in vitro viruses of the <i>Togaviridae</i> and <i>Flaviviridae</i> families which consists in: providing at least a fraction of LVP's obtained from serum or plasma of a patient infected with at least a virus of the <i>Togaviridae</i> and <i>Flaviviridae</i> families; contacting said fraction, for a predetermined time interval in an appropriate culture medium, with permissive cells having an endocytosis path relayed by at least one receptor of the lipoproteins and modulated inter alia by an activating agent selected among an unsaturated fatty acid or an unsaturated fatty acid derivative comprising 16 to 20 carbon atoms or their mixture. The invention also concerns the uses of viral particles and polypeptides obtained by said method.
Selon le procédé de culture, de propagation et de réplicationin vitro de virus appartenant aux familles des Togaviridae et Flaviviridae, on dispose d'au moins une fraction de LUPs obtenue à partir de sérum ou de plasma d'un patient infecté par au moins un virus appartenant aux familles des Togaviridae et Flaviviridae, on met en contact ladite fraction, pendant un temps prédéterminé dans un milieu de culture approprié, avec des cellules permissives qui possèdent une voie d'endocytose relayée par au moins un récepteur des lipoprotéines et modulée entre autres par un agent activateur choisi parmi un acide gras insaturé ou un dérivé d'un acide gras insaturé comprenant de 16 à 20 atomes de carbone ou leur mélange. L'invention concerne aussi les applications des particules virales et polypeptides obtenus selon le procédé précité.
Andre Patrice
Komurian-Pradel Florence
Lotteau Vincent
Paranhos-Baccala Glaucia
Bio Merieux
Institut National de La Sante Et de La Recherche Medicale
Norton Rose Or S.e.n.c.r.l. S.r.l./llp
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