Method for in vitro mass culture of aloe vera

Details Method for in vitro mass culture of aloe vera Method for in vitro mass culture of aloe vera

A - Human Necessities

01

H

A01H 4/00 (2006.01)

Patent

CA 2647851

The present invention provides a method for producing a true-to-type clone of an Aloe b.alpha.rb.alpha.densis mother plant by selecting an Aloe b.alpha.rb.alpha.densis mother plant; isolating a meristematic explant from the plant; culturing the meristematic explant in initiation medium to generate shoots, where the media lacks hormones; culturing the shoots in proliferation and elongation medium to generate elongated shoots, where the proliferation and elongation media comprises benzyl adenine (BA) and indole butyric acid (IBA); culturing the elongated shoots in rooting medium to generate plantlets, where the media lacks hormones; and culturing the plantlets to product a true-to-type clone of the Aloe b.alpha.rb.alpha.densis mother plant.

La présente invention a pour objet un procédé permettant la production d'un clone conforme au type d'un plant-mère d'Aloe b.alpha.rb.alpha.densis par la sélection d'un plant-mère d'Aloe b.alpha.rb.alpha.densis, l'isolement d'un explant méristématique du plant, la culture de l'explant dans un milieu d'initiation pour produire des pousses, le milieu ne contenant aucune hormone, la culture des pousses dans un milieu de prolifération et d'élongation pour produire des pousses allongées, le milieu comprenant de la benzyladénine (BA) et de l'acide indole butyrique (IBA), la culture des pousses allongées dans un milieu d'enracinement pour produire des plantules, le milieu ne contenant aucune hormone, et la culture des plantules pour obtenir un clone conforme au type du plant-mère d'Aloe b.alpha.rb.alpha.densis.

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