Method for in vitro propagation and maintenance of cells

C - Chemistry – Metallurgy – 12 – N

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195/46

C12N 5/00 (2006.01) C12M 3/06 (2006.01) C12N 1/10 (2006.01)

Patent

CA 1107211

METHOD FOR IN VITRO PROPAGATION AND MAINTENANCE OF CELLS ABSTRACT OF THE DISCLOSURE The propagation and/or maintenance of living cells in vitro is accomplished by attaching and growing cells on one side or surface of a hollow fiber membrane. The attached cells are propagated and/or maintained by passing oxygen through the membrane from the other side and into contact with the cells and simultaneously incubating the cells in a nutrient medium. This method is employed in tissue and cell culturing of altered and unaltered cells which attach to a surface for maintenance and growth. By: Jacques J. Delente

203742

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