G - Physics – 01 – N
Patent
G - Physics
01
N
G01N 33/15 (2006.01) A61K 49/00 (2006.01)
Patent
CA 2525624
As per invention embodiment, the method provides Deuterium Depleted Water administering to tested animals before and after tumoral grafting with animal grafts and it takes the following steps: A) Deuterium Depleted Water administering to Wistar outbred rats by diet, with concentrations of 25 ppm D2, 60 ppm D2, and 100 ppm D2, over a period of 60 days, simultaneously to dieting a control group of animals with water having 150 ppm content of Deuterium (tap water), over the same period of time. B) Viability determination for the tumor cells to be grafted, using tripan blue C) Grafting of the animals in the experimental group and the control animals in the 60th day, subcutaneous, with 1 x 107 malign tumor cells in 0.5 cc of normal saline solution of 256 Walker sarcoma (the solid tumor), and T8 Guérinlymphotropic epitelioma (the solid tumor) both of them having cells with a viability over 98%. D) Continuously and long-term administering, by diet, of Deuterium Depleted Water, with concentrations of 25 ppm, 60 ppm, 100 ppm Deuterium, period over which the followings are to be done: a. Starting with the 4thpost- graft day the tumor nodules measurement and examination is performed on each 2- 3 days; b. Monitoring of animals' physiological condition by weekly weighing, monitoring their food and water consumption, notifying the toxic condition occurrence c. After 60 days, when all the animals in control group are dead, preferable between the 160th and 200th day after graft, the effect produced by administering of established concentrations of Deuterium Depleted Water is observed on the surviving animals homeostasis from experimental groups, respectively the way how humoral immune system and cellular immune system of these animals has been influenced, by performing of a series of examination on immunological condition of the animals, namely: leucocytes formula test to establish lymphocytes and blastic cells levels; hematopoietic marrow tests to establish the plasmocytes and NK-K cells levels. Also, was established the level of the competent immunologic cells in the lymph nods of the tumoral graft zona. E) Determination of efficient concentration of Deuterium Depleted Water for tested surviving animals depending on new homeostasis occurrence, and on the results obtained related to, tumoral regression, as well as on cancer curing cases. This method determines the 60 ppm concentration of Deuterium Depleted Water to be the most efficient for cancer therapy and prophylaxis by continuously and long-term administering of this type of water in daily diet.
L'invention concerne un procédé consistant à administrer de l'eau appauvrie en deutérium à des animaux avant et après greffe. Ledit procédé consiste à administrer de l'eau appauvrie en deutérium à des rats croisés, ayant des concentrations de 25 ppm D2, 60 ppm D2, et 100 ppm D2 sur une période de 60 jours, et à administrer de l'eau ayant une concentration de 150 ppm D2 (eau courante) à un groupe d'animaux témoins, sur la même période de 60 jours ; à déterminer la viabilité des cellules tumorales à greffer à l'aide de bleu tripane ; à greffer les animaux de test et les animaux témoins le 60ème jour ; à administrer de l'eau appauvrie en deutérium ayant des concentrations de 25 ppm D2, 60 ppm D2, et 100 ppm D2, avec (a) mesure et examen tous les 2-3 jours à partir du 4ème jour post-greffe, (b) contrôle de l'état physiologique des animaux par pesée hebdomadaire, et de leur alimentation, et notification d'états de toxicité, (c) après 60 jours, lorsque tous les animaux du groupe témoin sont morts, de préférence entre le 160ème et le 200ème jour, observation de l'administration d'eau appauvrie en deutérium à des concentrations définies, sur l'homéostasie des animaux, c.-à-d. l'influence sur le système immunitaire humoral et le système immunitaire cellulaire, par tests de leucocytes visant à établir les taux cellulaires de lymphocytes et blastiques, et tests de moelle hématopoïétique visant à établir les taux cellulaires de plasmocytes et de NKK, et les taux de cellules immunologiques compétentes dans les noeuds lymphatiques de la zone de greffe tumorale ; et, à déterminer la concentration en eau appauvrie en deutérium chez les animaux survivants, en fonction de l'homéostasie, de la régression tumorale et des cas de guérison. Finalement, l'administration d'eau appauvrie en deutérium à une concentration de 60 ppm D2, est la plus efficace pour le traitement et la prophylaxie du cancer par administration continue, quotidienne, à long terme.
Balanescu Ion
Balint Emilia
Begu Daniela
Cinca Sabin
Comisel Virgiliu
Gowling Lafleur Henderson Llp
Institutul Oncologic "prof. Dr. Al. Trestioreanu" Bucuresti
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