C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/87 (2006.01) A01H 4/00 (2006.01) A01H 5/00 (2006.01) A01H 7/00 (2006.01) C07K 14/395 (2006.01) C12N 5/10 (2006.01) C12N 15/82 (2006.01)
Patent
CA 2147006
Disclosed is a method of making recombinant plant cells having reduced variability of expression and increased levels of expression of foreign genes therein. The method comprises (a) providing a plant cell capable of regeneration; (b) transforming the plant cell with a DNA construct comprising an expression cassette, which construct comprises, in the 5' to 3' direction, a transcription initiation region, a structural gene positioned downstream from the transcription initiation region and operatively associated therewith, and a scaffold attachment region positioned either 5' to the transcription initiation region or 3' to the structural gene, the expression cassette subject to the proviso that T-DNA borders are excluded therefrom. DNA constructs and vectors employed in carrying out the foregoing method are also disclosed, along with plant cells and plants produced thereby.
Allen George C.
Childs Lisa C.
Hall Gerald E. Jr.
Spiker Steven L.
Thompson William F.
North Carolina State University
Sim & Mcburney
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