Method for increasing storage lipid content in plant seed

A - Human Necessities – 01 – H

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A01H 5/00 (2006.01) A01H 5/10 (2006.01) C12N 9/12 (2006.01) C12N 15/11 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2196519

In order to obtain a crop which provides an increased storage lipid content in its seed, the activity of cytosolic pyruvate kinase of a seed is substantially lowered as compared with the endogenous activity of pyruvate kinase in the plant seed. Preferably, the activity of phosphoenolpyruvate carboxylase is substantially lowered as compared with the endogenous activity of phosphoenolpyruvate carboxylase in the seed. Thus the storage lipid content in the plant seed is increased.

Afin d'obtenir une culture pouvant stocker une quantité accrue de lipides dans la graine, on réduit de façon substantielle l'activité de la pyruvate-kinase cytosolique de la graine comparativement à l'activité endogène normale de cette enzyme dans la graine de la plante. De préférence, on réduit également l'activité de la phosphoénolpyruvate-carboxylase de façon substantielle dans la graine. On augmente ainsi la capacité de stockage de lipides dans la graine de la plante.

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